Hotspot

trnS-GCU-trnG-GCC

LSC · 8575-9399 · 825 bp

Back to Chimonanthus

Pi 0.00574

MarkerSeek score 49.80

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 825
variable_sites: 11
pi: 0.00574
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	CAATCCGTCGCTTTAGTCCA	ACCGAAAGAACCCTTATCCA	50.0	45.0	58.0	56.1	931-933 bp	1.0	66.0
trnS-GCU-trnG-GCC_p2	AATCCGTCGCTTTAGTCCAC	ACCGAAAGAACCCTTATCCA	50.0	45.0	58.0	56.1	930-932 bp	1.0	66.0
trnS-GCU-trnG-GCC_p3	GATCAGAGGGAACGGAGAGA	ACCGAAAGAACCCTTATCCA	55.0	45.0	57.9	56.1	996-998 bp	1.0	65.5
trnS-GCU-trnG-GCC_p4	ATCAGAGGGAACGGAGAGAG	ACCGAAAGAACCCTTATCCA	55.0	45.0	57.9	56.1	995-997 bp	1.0	65.5
trnS-GCU-trnG-GCC_p5	GGAACGGAGAGAGAGGGATT	ACCGAAAGAACCCTTATCCA	55.0	45.0	58.2	56.1	988-990 bp	1.0	64.3