Hotspot

rps16-trnQ-UUG

LSC · 6114-7250 · 1137 bp

Back to Chimonanthus

Pi 0.00457

MarkerSeek score 57.80

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1137
variable_sites: 13
pi: 0.00457
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1196-1229 bp	1.0	80.2
rps16-trnQ-UUG_p2	TGCAAGAATCCACAACCGAT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.8	58.1	1226-1259 bp	1.0	79.7
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	45.0	55.0	58.1	57.8	1200-1233 bp	1.0	79.2
rps16-trnQ-UUG_p4	TGCAAGAATCCACAACCGAT	CTCGGAGGTTCGAATCCTTC	45.0	55.0	57.8	57.8	1230-1263 bp	1.0	78.6
rps16-trnQ-UUG_p5	AATGGTGGGTGCAAGAATCC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.4	58.1	1235-1268 bp	1.0	77.8