Hotspot

ycf1

IRb · 107931-108092 · 162 bp

Back to Chiloschista

Pi 0.0685

MarkerSeek score 65.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 162
variable_sites: 25
pi: 0.0685
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GCTTCCTTCGTAACTCCAGG	CTTTTTGACTGTCCGGTGGA	55.0	50.0	58.0	58.0	927-953 bp	1.0	89.0
ycf1_p2	AAAGCGAGCCCTTTCATTCA	CTTTTTGACTGTCCGGTGGA	45.0	50.0	58.1	58.0	1119-1145 bp	1.0	87.5
ycf1_p3	AAGCGAGCCCTTTCATTCAA	CTTTTTGACTGTCCGGTGGA	45.0	50.0	58.1	58.0	1118-1144 bp	1.0	87.5
ycf1_p4	TCAAAGCGAGCCCTTTCATT	CTTTTTGACTGTCCGGTGGA	45.0	50.0	58.1	58.0	1121-1147 bp	1.0	87.5
ycf1_p5	GGCAGAATAGCAGAGCAAGT	CTTTTTGACTGTCCGGTGGA	50.0	50.0	58.0	58.0	1252-1416 bp	1.0	87.1