Hotspot

trnN-GUU-trnR-ACG

IRa · 119136-119545 · 410 bp

Back to Chiloschista

Pi 0.00537

MarkerSeek score 64.20

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 410
variable_sites: 5
pi: 0.00537
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	CGGTTGGCTGTTAACTGACT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.1	57.8	521-669 bp	1.0	80.3
trnN-GUU-trnR-ACG_p2	GCTGTTAACTGACTGGTCGT	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.8	57.8	515-663 bp	1.0	80.1
trnN-GUU-trnR-ACG_p3	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	502-650 bp	1.0	80.1
trnN-GUU-trnR-ACG_p4	CGGTTGGCTGTTAACTGACT	TTTGAAGGTACTCCCGGGAA	50.0	50.0	58.1	58.3	575-723 bp	1.0	79.7
trnN-GUU-trnR-ACG_p5	CGGTTGGCTGTTAACTGACT	TTTTGAAGGTACTCCCGGGA	50.0	50.0	58.1	58.3	576-724 bp	1.0	79.7