Hotspot

ndhE-ndhG

SSC · 111757-111942 · 186 bp

Back to Chiloschista

Pi 0.0188

MarkerSeek score 61.40

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 186
variable_sites: 8
pi: 0.0188
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	ACCGATAGAAAAGAAATAAACACT	AGGTGCTATTACTATGACTTGTCA	29.2	37.5	54.6	57.2	262-277 bp	0.8	37.1
ndhE-ndhG_p2	ACCGATAGAAAAGAAATAAACACTT	AGGTGCTATTACTATGACTTGTCA	28.0	37.5	55.3	57.2	262-277 bp	0.8	37.1
ndhE-ndhG_p3	ACCGATAGAAAAGAAATAAACACT	GGTGCTATTACTATGACTTGTCA	29.2	39.1	54.6	55.8	261-276 bp	0.8	37.1
ndhE-ndhG_p4	CCATAGATACCGATAGAAAAGA	AGGTGCTATTACTATGACTTGTCA	36.4	37.5	52.4	57.2	270-285 bp	0.8	36.9
ndhE-ndhG_p5	CCATAGATACCGATAGAAAAGAA	AGGTGCTATTACTATGACTTGTCA	34.8	37.5	53.2	57.2	270-285 bp	0.8	36.9