Hotspot

psaC-ndhE

SSC · 110826-111540 · 715 bp

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Pi 0.0143

MarkerSeek score 57.60

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 715
variable_sites: 22
pi: 0.0143
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	AATACATCCGTGGGACAAGC	CGAAGCATGGCTAGAACACT	50.0	50.0	58.0	58.0	648-977 bp	1.0	86.1
psaC-ndhE_p2	GGACAAGCGGATTCACATCT	CGAAGCATGGCTAGAACACT	50.0	50.0	58.0	58.0	747-1076 bp	1.0	85.9
psaC-ndhE_p3	TATACCAGAAAAGCCCGTGC	CGAAGCATGGCTAGAACACT	50.0	50.0	58.0	58.0	931-1260 bp	1.0	85.8
psaC-ndhE_p4	AGCTCGAACACATTGGGTAC	CGAAGCATGGCTAGAACACT	50.0	50.0	57.9	58.0	631-960 bp	1.0	85.7
psaC-ndhE_p5	CCATCGGCATTCTTTCTGGA	CGAAGCATGGCTAGAACACT	50.0	50.0	58.0	58.0	1293-1620 bp	1.0	85.5