Hotspot

ycf1-rpl32

SSC · 108093-108478 · 386 bp

Back to Chiloschista

Pi 0.0422

MarkerSeek score 74.30

Primer available yes

Label ycf1-rpl32

Feature Metadata

label_name: ycf1-rpl32
feature_type: igs
region: SSC
length_bp: 386
variable_sites: 34
pi: 0.0422
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rpl32_p1	GCTTCCTTCGTAACTCCAGG	CTTTTTGACTGTCCGGTGGA	55.0	50.0	58.0	58.0	927-953 bp	1.0	89.0
ycf1-rpl32_p2	AAAGCGAGCCCTTTCATTCA	CTTTTTGACTGTCCGGTGGA	45.0	50.0	58.1	58.0	1119-1145 bp	1.0	87.5
ycf1-rpl32_p3	AAGCGAGCCCTTTCATTCAA	CTTTTTGACTGTCCGGTGGA	45.0	50.0	58.1	58.0	1118-1144 bp	1.0	87.5
ycf1-rpl32_p4	GGCAGAATAGCAGAGCAAGT	CTTTTTGACTGTCCGGTGGA	50.0	50.0	58.0	58.0	1252-1416 bp	1.0	87.1
ycf1-rpl32_p5	CGGTCTTTGTGATCGGACTT	CTTTTTGACTGTCCGGTGGA	50.0	50.0	57.9	58.0	616-628 bp	0.8	75.8