Hotspot

trnR-ACG-trnN-GUU

IRb · 107132-107541 · 410 bp

Back to Chiloschista

Pi 0.00537
MarkerSeek score 64.20
Primer available yes
Label trnR-ACG-trnN-GUU

Feature Metadata

label_name
trnR-ACG-trnN-GUU
feature_type
igs
region
IRb
length_bp
410
variable_sites
5
pi
0.00537
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-ACG-trnN-GUU_p1 CAGAGGATTAGAGCACGTGG CGGTTGGCTGTTAACTGACT 55.0 50.0 57.8 58.1 521-669 bp 1.0 80.3
trnR-ACG-trnN-GUU_p2 CAGAGGATTAGAGCACGTGG GCTGTTAACTGACTGGTCGT 55.0 50.0 57.8 57.8 515-663 bp 1.0 80.1
trnR-ACG-trnN-GUU_p3 CAGAGGATTAGAGCACGTGG TGGTCGTAGGTTCGAATCCT 55.0 50.0 57.8 58.2 502-650 bp 1.0 80.1
trnR-ACG-trnN-GUU_p4 TTTGAAGGTACTCCCGGGAA CGGTTGGCTGTTAACTGACT 50.0 50.0 58.3 58.1 575-723 bp 1.0 79.7
trnR-ACG-trnN-GUU_p5 TTTTGAAGGTACTCCCGGGA CGGTTGGCTGTTAACTGACT 50.0 50.0 58.3 58.1 576-724 bp 1.0 79.7