Hotspot

ndhJ-trnV-UAC

LSC · 49245-50037 · 793 bp

Back to Chiloschista

Pi 0.0157

MarkerSeek score 61.60

Primer available yes

Label ndhJ-trnV-UAC

Feature Metadata

label_name: ndhJ-trnV-UAC
feature_type: igs
region: LSC
length_bp: 793
variable_sites: 28
pi: 0.0157
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-trnV-UAC_p1	GCACACTGGGAGCGTAAATA	TGAATCAGAGCACATGGAGC	50.0	50.0	58.0	58.0	1115-1928 bp	1.0	85.9
ndhJ-trnV-UAC_p2	CCTCGTGTCACCAGTTCAAA	TGAATCAGAGCACATGGAGC	50.0	50.0	58.1	58.0	1339-2152 bp	1.0	85.2
ndhJ-trnV-UAC_p3	AATCTGGTTCCTGACACGTG	TGAATCAGAGCACATGGAGC	50.0	50.0	57.8	58.0	1321-2134 bp	1.0	84.3
ndhJ-trnV-UAC_p4	CTGGTTCCTGACACGTGAAT	TGAATCAGAGCACATGGAGC	50.0	50.0	57.8	58.0	1318-2131 bp	1.0	84.3
ndhJ-trnV-UAC_p5	GCACACTGGGAGCGTAAATA	ATCCGGTTCAAGTGCGAATT	50.0	45.0	58.0	58.2	1398-2211 bp	1.0	84.1