Hotspot

pafI-trnS-GGA

LSC · 45089-45670 · 582 bp

Back to Chiloschista

Pi 0.012

MarkerSeek score 62.70

Primer available yes

Label pafI-trnS-GGA

Feature Metadata

label_name: pafI-trnS-GGA
feature_type: igs
region: LSC
length_bp: 582
variable_sites: 17
pi: 0.012
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
pafI-trnS-GGA_p1	TTTTCCCCTGAAGTTGTCGG	TTCCAATGCTACGCCTTGAA	50.0	45.0	58.0	57.8	716-718 bp	1.0	80.4
pafI-trnS-GGA_p2	TTTTCCCCTGAAGTTGTCGG	CGGAGAGAGAGGGATTCGAA	50.0	55.0	58.0	58.3	765-767 bp	1.0	79.2
pafI-trnS-GGA_p3	CCCCTGAAGTTGTCGGAATT	TTCCAATGCTACGCCTTGAA	50.0	45.0	57.8	57.8	712-714 bp	1.0	79.0
pafI-trnS-GGA_p4	CCCCTGAAGTTGTCGGAATT	CGGAGAGAGAGGGATTCGAA	50.0	55.0	57.8	58.3	761-763 bp	1.0	77.8
pafI-trnS-GGA_p5	TTTTCCCCTGAAGTTGTCGG	AGTTCCAATGCTACGCCTTG	50.0	50.0	58.0	58.5	718-720 bp	1.0	77.5