Hotspot

rpoB-trnC-GCA

LSC · 27311-28731 · 1421 bp

Back to Chiloschista

Pi 0.0196

MarkerSeek score 77.10

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1421
variable_sites: 63
pi: 0.0196
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AATTCTTCCGTCAAGCCCTG	CCGGGGATAAAGGATTTGCA	50.0	50.0	58.2	57.9	1396-1671 bp	1.0	85.3
rpoB-trnC-GCA_p2	AATTCTTCCGTCAAGCCCTG	ACCGGGGATAAAGGATTTGC	50.0	50.0	58.2	57.9	1397-1672 bp	1.0	85.3
rpoB-trnC-GCA_p3	AATTCTTCCGTCAAGCCCTG	TCGAACCGGGGATAAAGGAT	50.0	50.0	58.2	58.2	1401-1676 bp	1.0	84.2
rpoB-trnC-GCA_p4	AATTCTTCCGTCAAGCCCTG	TTCGAACCGGGGATAAAGGA	50.0	50.0	58.2	58.4	1402-1677 bp	1.0	82.4
rpoB-trnC-GCA_p5	AAATTCTTCCGTCAAGCCCT	CCGGGGATAAAGGATTTGCA	45.0	50.0	57.4	57.9	1397-1672 bp	1.0	82.0