Hotspot

psbI-trnS-GCU

LSC · 8139-8316 · 178 bp

Back to Chiloschista

Pi 0.0192

MarkerSeek score 65.20

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 178
variable_sites: 8
pi: 0.0192
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	CTTATCTAACGATCCGGGGC	TGGACTAAAGCGTCGGATTG	55.0	50.0	57.6	58.0	283-294 bp	1.0	83.2
psbI-trnS-GCU_p2	CTTATCTAACGATCCGGGGC	GTGGACTAAAGCGTCGGATT	55.0	50.0	57.6	58.0	284-295 bp	1.0	83.2
psbI-trnS-GCU_p3	GTAATCCTGGGCGTGAAGAA	TGGACTAAAGCGTCGGATTG	50.0	50.0	57.6	58.0	263-274 bp	1.0	83.2
psbI-trnS-GCU_p4	GTAATCCTGGGCGTGAAGAA	GTGGACTAAAGCGTCGGATT	50.0	50.0	57.6	58.0	264-275 bp	1.0	83.1
psbI-trnS-GCU_p5	CTTATCTAACGATCCGGGGC	GGGTTCGAATCCCTCTCTCT	55.0	55.0	57.6	58.2	233-244 bp	1.0	82.0