Hotspot

ycf3

LSC · 43196-45174 · 1979 bp

Back to Chikusichloa

Pi 0.00152

MarkerSeek score 57.30

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 1979
variable_sites: 3
pi: 0.00152
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	TCAACAACCAAACCCCCATT	TCCATTTAGAGCATATGGATCGA	45.0	39.1	57.8	57.0	2129-2130 bp	1.0	46.6
ycf3_p2	GATCAACAACCAAACCCCCA	TCCATTTAGAGCATATGGATCGA	50.0	39.1	58.3	57.0	2131-2132 bp	1.0	45.8
ycf3_p3	TGGATCAACAACCAAACCCC	TCCATTTAGAGCATATGGATCGA	50.0	39.1	58.3	57.0	2133-2134 bp	1.0	45.8
ycf3_p4	ATCAACAACCAAACCCCCAT	TCCATTTAGAGCATATGGATCGA	45.0	39.1	57.6	57.0	2130-2131 bp	1.0	44.8
ycf3_p5	TCAACAACCAAACCCCCATT	CCATTTAGAGCATATGGATCGA	45.0	40.9	57.8	55.6	2128-2129 bp	1.0	43.2