Hotspot

trnG-UCC

LSC · 13005-13052 · 48 bp

Back to Chikusichloa

Pi 0.0208
MarkerSeek score 64.50
Primer available yes
Label trnG-UCC

Feature Metadata

label_name
trnG-UCC
feature_type
tRNA
region
LSC
length_bp
48
variable_sites
1
pi
0.0208
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC_p1 ACTGCTCTACTCCGCTCTAG ACCCATAAAGGAGCCGAATG 55.0 50.0 58.0 57.7 242 bp 1.0 77.2
trnG-UCC_p2 CTCTACTCCGCTCTAGAGGG ACCCATAAAGGAGCCGAATG 60.0 50.0 57.8 57.7 238 bp 1.0 76.1
trnG-UCC_p3 CTGCTCTACTCCGCTCTAGA ACCCATAAAGGAGCCGAATG 55.0 50.0 57.8 57.7 241 bp 1.0 75.6
trnG-UCC_p4 TGCTCTACTCCGCTCTAGAG ACCCATAAAGGAGCCGAATG 55.0 50.0 57.8 57.7 240 bp 1.0 75.6
trnG-UCC_p5 ACTGCTCTACTCCGCTCTAG CCCATAAAGGAGCCGAATGA 55.0 50.0 58.0 57.4 241 bp 1.0 74.9
trnG-UCC_p1 ACTGCTCTACTCCGCTCTAG TGGGGTGGTAAGAAGAGGTT 55.0 50.0 58.0 57.9 1066 bp 1.0 78.8
trnG-UCC_p2 ACTGCTCTACTCCGCTCTAG TTGGGGTGGTAAGAAGAGGT 55.0 50.0 58.0 57.9 1067 bp 1.0 78.8
trnG-UCC_p3 CTCTACTCCGCTCTAGAGGG TGGGGTGGTAAGAAGAGGTT 60.0 50.0 57.8 57.9 1062 bp 1.0 77.7
trnG-UCC_p4 CTCTACTCCGCTCTAGAGGG TTGGGGTGGTAAGAAGAGGT 60.0 50.0 57.8 57.9 1063 bp 1.0 77.7
trnG-UCC_p5 TGCTCTACTCCGCTCTAGAG TGGGGTGGTAAGAAGAGGTT 55.0 50.0 57.8 57.9 1064 bp 1.0 77.1