Hotspot

infA

LSC · 77952-78257 · 306 bp

Back to Chikusichloa

Pi 0

MarkerSeek score 20.90

Primer available yes

Label infA

Feature Metadata

label_name: infA
feature_type: gene
region: LSC
length_bp: 306
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 20.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA_p1	TCCACTACCAGCACCCTTTA	TTAAAGCGTATCAGCCGACC	50.0	50.0	58.0	58.1	1308 bp	1.0	79.4
infA_p2	TTGCAAACCCACTGTACGAA	TTAAAGCGTATCAGCCGACC	45.0	50.0	58.0	58.1	1272 bp	1.0	79.3
infA_p3	GCAGCATCTCTTCCACTACC	TTAAAGCGTATCAGCCGACC	55.0	50.0	58.1	58.1	1319 bp	1.0	79.2
infA_p4	GCATCTCTTCCACTACCAGC	TTAAAGCGTATCAGCCGACC	55.0	50.0	58.1	58.1	1316 bp	1.0	79.2
infA_p5	TTTGGACATTTTCGCGGGTA	TTAAAGCGTATCAGCCGACC	45.0	50.0	58.1	58.1	882 bp	1.0	78.9