Hotspot

trnL-CAA-trnI-CAU

IRa · 132442-133946 · 1505 bp

Back to Chikusichloa

Pi 0.00199

MarkerSeek score 59.90

Primer available yes

Label trnL-CAA-trnI-CAU

Feature Metadata

label_name: trnL-CAA-trnI-CAU
feature_type: igs
region: IRa
length_bp: 1505
variable_sites: 3
pi: 0.00199
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-trnI-CAU_p1	AAATGGTAGACACGCGAGAC	GTTGGGCGCTTTAACCATTC	50.0	50.0	58.0	58.0	1606 bp	1.0	80.0
trnL-CAA-trnI-CAU_p2	TGAAATGGTAGACACGCGAG	GTTGGGCGCTTTAACCATTC	50.0	50.0	58.0	58.0	1608 bp	1.0	80.0
trnL-CAA-trnI-CAU_p3	TGGTGAAATGGTAGACACGC	GTTGGGCGCTTTAACCATTC	50.0	50.0	58.2	58.0	1611 bp	1.0	78.5
trnL-CAA-trnI-CAU_p4	GAAATGGTAGACACGCGAGA	GTTGGGCGCTTTAACCATTC	50.0	50.0	57.7	58.0	1607 bp	1.0	77.9
trnL-CAA-trnI-CAU_p5	GTTCGAGTCCTCTTCAAGGC	GTTGGGCGCTTTAACCATTC	55.0	50.0	58.3	58.0	1558 bp	1.0	77.8