Hotspot

psaC-ndhE

SSC · 110248-110726 · 479 bp

Back to Chikusichloa

Pi 0.00626

MarkerSeek score 59.80

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 479
variable_sites: 3
pi: 0.00626
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	GCTTTACATCCATCCCAGGG	TTTTGTTATAGCCCTTGCGG	55.0	45.0	58.0	56.4	701 bp	1.0	67.4
psaC-ndhE_p2	GGCTTTACATCCATCCCAGG	TTTTGTTATAGCCCTTGCGG	55.0	45.0	58.0	56.4	702 bp	1.0	67.4
psaC-ndhE_p3	GTTGGACAAGCTCGTACACA	TTTTGTTATAGCCCTTGCGG	50.0	45.0	58.1	56.4	662 bp	1.0	66.4
psaC-ndhE_p4	TGTTGGACAAGCTCGTACAC	TTTTGTTATAGCCCTTGCGG	50.0	45.0	58.1	56.4	663 bp	1.0	66.4
psaC-ndhE_p5	CTGTTGGACAAGCTCGTACA	TTTTGTTATAGCCCTTGCGG	50.0	45.0	57.9	56.4	664 bp	1.0	66.3