Hotspot

trnI-CAU-trnL-CAA

IRb · 84932-86436 · 1505 bp

Back to Chikusichloa

Pi 0.00199

MarkerSeek score 59.90

Primer available yes

Label trnI-CAU-trnL-CAA

Feature Metadata

label_name: trnI-CAU-trnL-CAA
feature_type: igs
region: IRb
length_bp: 1505
variable_sites: 3
pi: 0.00199
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-trnL-CAA_p1	GTTGGGCGCTTTAACCATTC	AAATGGTAGACACGCGAGAC	50.0	50.0	58.0	58.0	1606 bp	1.0	80.0
trnI-CAU-trnL-CAA_p2	GTTGGGCGCTTTAACCATTC	TGAAATGGTAGACACGCGAG	50.0	50.0	58.0	58.0	1608 bp	1.0	80.0
trnI-CAU-trnL-CAA_p3	GTTGGGCGCTTTAACCATTC	TGGTGAAATGGTAGACACGC	50.0	50.0	58.0	58.2	1611 bp	1.0	78.5
trnI-CAU-trnL-CAA_p4	GTTGGGCGCTTTAACCATTC	GAAATGGTAGACACGCGAGA	50.0	50.0	58.0	57.7	1607 bp	1.0	77.9
trnI-CAU-trnL-CAA_p5	GTTGGGCGCTTTAACCATTC	GTTCGAGTCCTCTTCAAGGC	50.0	55.0	58.0	58.3	1558 bp	1.0	77.8