Hotspot

rpl20-rps12

LSC · 68384-69082 · 699 bp

Back to Chikusichloa

Pi 0.00143

MarkerSeek score 59.60

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 699
variable_sites: 1
pi: 0.00143
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	ACGCATTTTTGTTCGTCGTC	GAGGAACATGTGCTAGGGTG	45.0	55.0	58.0	58.0	776 bp	1.0	79.8
rpl20-rps12_p2	ACGCATTTTTGTTCGTCGTC	CGACATGCCAACGGTTAAAC	45.0	50.0	58.0	58.0	871 bp	1.0	79.7
rpl20-rps12_p3	ACGCATTTTTGTTCGTCGTC	GTCGAGGAACATGTGCTAGG	45.0	55.0	58.0	58.1	779 bp	1.0	79.4
rpl20-rps12_p4	GTTTGAGGCAAATGAACGCA	GAGGAACATGTGCTAGGGTG	45.0	55.0	57.9	58.0	791 bp	1.0	78.8
rpl20-rps12_p5	GTTTGAGGCAAATGAACGCA	CGACATGCCAACGGTTAAAC	45.0	50.0	57.9	58.0	886 bp	1.0	78.7