Hotspot

atpB-rbcL

LSC · 54525-55308 · 784 bp

Back to Chikusichloa

Pi 0.00383

MarkerSeek score 61.00

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 784
variable_sites: 3
pi: 0.00383
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GGGAAAAGTGACATCCAGCA	ACTCCGGGGTGTAGTAAGTC	50.0	55.0	58.1	58.2	977 bp	1.0	78.3
atpB-rbcL_p2	GGGAAAAGTGACATCCAGCA	CTCCGGGGTGTAGTAAGTCA	50.0	55.0	58.1	58.2	976 bp	1.0	78.3
atpB-rbcL_p3	GGGGGAAAAGTGACATCCAG	ACTCCGGGGTGTAGTAAGTC	55.0	55.0	58.2	58.2	979 bp	1.0	77.7
atpB-rbcL_p4	GGGGGAAAAGTGACATCCAG	CTCCGGGGTGTAGTAAGTCA	55.0	55.0	58.2	58.2	978 bp	1.0	77.7
atpB-rbcL_p5	GGGAAAAGTGACATCCAGCA	CGTACTCCGGGGTGTAGTAA	50.0	55.0	58.1	58.2	980 bp	1.0	77.5