Hotspot

orf448

Genome · 149625-150971 · 1347 bp

Back to Chiharaea

Pi 0.0119

MarkerSeek score 51.00

Primer available yes

Label orf448

Feature Metadata

label_name: orf448
feature_type: gene
region: Genome
length_bp: 1347
variable_sites: 16
pi: 0.0119
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf448_p1	ACCAACAATACCTGCTGCAA	AAGTCCAACCTTGCTCGTTT	45.0	45.0	58.0	57.9	2341 bp	1.0	80.2
orf448_p2	ACCAACAATACCTGCTGCAA	AGTCCAACCTTGCTCGTTTT	45.0	45.0	58.0	57.9	2340 bp	1.0	80.2
orf448_p3	ACCAACAATACCTGCTGCAA	GGCTGCTTGGTTTCTACGTA	45.0	50.0	58.0	57.9	2256 bp	1.0	80.0
orf448_p4	CTCGCTATAGAGGACCTCGT	AAGTCCAACCTTGCTCGTTT	55.0	45.0	57.8	57.9	2642 bp	1.0	78.9
orf448_p5	CTCGCTATAGAGGACCTCGT	AGTCCAACCTTGCTCGTTTT	55.0	45.0	57.8	57.9	2641 bp	1.0	78.9