Hotspot

chlB

Genome · 92561-96048 · 3488 bp

Back to Chiharaea

Pi 0.00803

MarkerSeek score 46.40

Primer available yes

Label chlB

Feature Metadata

label_name: chlB
feature_type: gene
region: Genome
length_bp: 3488
variable_sites: 28
pi: 0.00803
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlB_p1	ATTGTGAAAAATTTACTATGGAAC	TCGAGATAGACCTCTACTCA	25.0	45.0	52.3	53.5	200 bp	1.0	42.0
chlB_p2	ATTGTGAAAAATTTACTATGGAAC	TTCGAGATAGACCTCTACTCA	25.0	42.9	52.3	54.4	201 bp	1.0	42.0
chlB_p3	ATTGTGAAAAATTTACTATGGAAC	TTTCGAGATAGACCTCTACTCA	25.0	40.9	52.3	55.2	202 bp	1.0	42.0
chlB_p4	AATGCTTAATATTTTCTATGGAGT	TCGAGATAGACCTCTACTCA	25.0	45.0	52.3	53.5	243 bp	1.0	41.6
chlB_p5	AATGCTTAATATTTTCTATGGAGT	TTCGAGATAGACCTCTACTCA	25.0	42.9	52.3	54.4	244 bp	1.0	41.6