Hotspot

pgmA-ycf21

Genome · 168479-169395 · 917 bp

Back to Chiharaea

Pi 0.0144

MarkerSeek score 53.10

Primer available yes

Label pgmA-ycf21

Feature Metadata

label_name: pgmA-ycf21
feature_type: igs
region: Genome
length_bp: 917
variable_sites: 10
pi: 0.0144
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.76
markerseek_score: 53.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
pgmA-ycf21_p1	GGATTGGATCATTGGCCGAT	CCCGACTGGTTGCTTCTTTA	50.0	50.0	58.1	57.8	1601-1823 bp	1.0	82.6
pgmA-ycf21_p2	GGATTGGATCATTGGCCGAT	CGCAAAAGCTTCAGGGAGTA	50.0	50.0	58.1	58.2	1576-1798 bp	1.0	82.5
pgmA-ycf21_p3	GCCGATATAGCGCCAAGTAT	CCCGACTGGTTGCTTCTTTA	50.0	50.0	57.7	57.8	1587-1809 bp	1.0	81.0
pgmA-ycf21_p4	GCCGATATAGCGCCAAGTAT	CGCAAAAGCTTCAGGGAGTA	50.0	50.0	57.7	58.2	1562-1784 bp	1.0	80.9
pgmA-ycf21_p5	ATCATTGGCCGATATAGCGC	CCCGACTGGTTGCTTCTTTA	50.0	50.0	58.3	57.8	1594-1816 bp	1.0	80.6