Hotspot

rpl29-rpl16

Genome · 76538-76541 · 4 bp

Back to Chiharaea

Pi 0.25

MarkerSeek score 73.90

Primer available yes

Label rpl29-rpl16

Feature Metadata

label_name: rpl29-rpl16
feature_type: igs
region: Genome
length_bp: 4
variable_sites: 1
pi: 0.25
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl29-rpl16_p1	AACCGGCATATTGGGTGAAG	CAACACAGAGGACGTCTACG	50.0	55.0	58.2	58.0	999 bp	1.0	78.3
rpl29-rpl16_p2	AACCGGCATATTGGGTGAAG	CAGCTGAAACGAGAATGGGA	50.0	50.0	58.2	57.9	800 bp	1.0	77.8
rpl29-rpl16_p3	AACCGGCATATTGGGTGAAG	GGTGCACCAGAATATTGGGT	50.0	50.0	58.2	57.9	771 bp	1.0	77.4
rpl29-rpl16_p4	AACCGGCATATTGGGTGAAG	TGCAAAGCAAGCCATGAAAC	50.0	45.0	58.2	58.1	694 bp	0.5	57.0
rpl29-rpl16_p5	AACCGGCATATTGGGTGAAG	GCAAAGCAAGCCATGAAACT	50.0	45.0	58.2	57.8	693 bp	0.5	56.8