Hotspot

trnS(gct)-trnD(gtc)

Genome · 59824-59884 · 61 bp

Back to Chiharaea

Pi 0.0328

MarkerSeek score 67.50

Primer available yes

Label trnS(gct)-trnD(gtc)

Feature Metadata

label_name: trnS(gct)-trnD(gtc)
feature_type: igs
region: Genome
length_bp: 61
variable_sites: 2
pi: 0.0328
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS(gct)-trnD(gtc)_p1	GAGAGATGGCTGAGTGGTTG	GGGCGATGCTCTAACCAATT	55.0	50.0	58.0	58.3	184 bp	1.0	78.1
trnS(gct)-trnD(gtc)_p2	TGAAAGCGGCAGATTGCTAA	GGGCGATGCTCTAACCAATT	45.0	50.0	58.2	58.3	166 bp	1.0	77.0
trnS(gct)-trnD(gtc)_p3	TTGAAAGCGGCAGATTGCTA	GGGCGATGCTCTAACCAATT	45.0	50.0	58.2	58.3	167 bp	1.0	77.0
trnS(gct)-trnD(gtc)_p4	GAGAGATGGCTGAGTGGTTG	ACCAATTGAACTACGGTCCC	55.0	50.0	58.0	57.5	171 bp	1.0	76.9
trnS(gct)-trnD(gtc)_p5	TGAAAGCGGCAGATTGCTAA	ACCAATTGAACTACGGTCCC	45.0	50.0	58.2	57.5	153 bp	1.0	75.7