Hotspot

trnY(gta)-trnT(ggt)

Genome · 55322-55346 · 25 bp

Back to Chiharaea

Pi 0.04

MarkerSeek score 67.80

Primer available yes

Label trnY(gta)-trnT(ggt)

Feature Metadata

label_name: trnY(gta)-trnT(ggt)
feature_type: igs
region: Genome
length_bp: 25
variable_sites: 1
pi: 0.04
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY(gta)-trnT(ggt)_p1	ACTGTAAATCCGCTGGCAAT	TCTGCCATTGAGCTACAAGG	45.0	50.0	57.9	57.6	98 bp	1.0	76.5
trnY(gta)-trnT(ggt)_p2	CTGTAAATCCGCTGGCAATG	TCTGCCATTGAGCTACAAGG	50.0	50.0	57.5	57.6	97 bp	1.0	73.7
trnY(gta)-trnT(ggt)_p3	GACTGTAAATCCGCTGGCAA	TCTGCCATTGAGCTACAAGG	50.0	50.0	58.6	57.6	99 bp	1.0	73.1
trnY(gta)-trnT(ggt)_p4	ATTCTTGATAGGGTCGGTGC	TCTGCCATTGAGCTACAAGG	50.0	50.0	57.4	57.6	139 bp	1.0	72.3
trnY(gta)-trnT(ggt)_p5	TAATGGGGGCGGACTGTAAA	TCTGCCATTGAGCTACAAGG	50.0	50.0	58.7	57.6	110 bp	1.0	71.8