Hotspot

trnF(gaa)-clpC

Genome · 51983-52054 · 72 bp

Back to Chiharaea

Pi 0.0556

MarkerSeek score 69.90

Primer available yes

Label trnF(gaa)-clpC

Feature Metadata

label_name: trnF(gaa)-clpC
feature_type: igs
region: Genome
length_bp: 72
variable_sites: 4
pi: 0.0556
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF(gaa)-clpC_p1	TGGTAGAGCAGTGGACTGAA	AGGCGAAAAATTGGAATTACT	50.0	33.3	58.0	54.0	160 bp	1.0	42.3
trnF(gaa)-clpC_p2	TTGGTAGAGCAGTGGACTGA	AGGCGAAAAATTGGAATTACT	50.0	33.3	58.0	54.0	161 bp	1.0	42.3
trnF(gaa)-clpC_p3	TCAGTTGGTAGAGCAGTGGA	AGGCGAAAAATTGGAATTACT	50.0	33.3	58.0	54.0	165 bp	1.0	42.2
trnF(gaa)-clpC_p4	AGTTGGTAGAGCAGTGGACT	AGGCGAAAAATTGGAATTACT	50.0	33.3	58.0	54.0	163 bp	1.0	42.2
trnF(gaa)-clpC_p5	ATCATAAGGCCGGGATAGCT	AGGCGAAAAATTGGAATTACT	50.0	33.3	58.0	54.0	184 bp	1.0	41.8