Hotspot

rpl1-rpl11

Genome · 47443-47465 · 23 bp

Back to Chiharaea

Pi 0.0435

MarkerSeek score 77.50

Primer available yes

Label rpl1-rpl11

Feature Metadata

label_name: rpl1-rpl11
feature_type: igs
region: Genome
length_bp: 23
variable_sites: 1
pi: 0.0435
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl1-rpl11_p1	GCTTGTAACGACGAGAAGACT	AGCAGCCACAAAAATACCAGA	47.6	42.9	58.1	58.1	154 bp	1.0	63.0
rpl1-rpl11_p2	TGCTTGTAACGACGAGAAGA	AGCAGCCACAAAAATACCAGA	45.0	42.9	56.9	58.1	155 bp	1.0	62.9
rpl1-rpl11_p3	GCTTGTAACGACGAGAAGACT	GCAGCCACAAAAATACCAGA	47.6	45.0	58.1	56.6	153 bp	1.0	60.8
rpl1-rpl11_p4	TGCTTGTAACGACGAGAAGA	GCAGCCACAAAAATACCAGA	45.0	45.0	56.9	56.6	154 bp	1.0	60.8
rpl1-rpl11_p5	TGCTTGTAACGACGAGAAGAC	AGCAGCCACAAAAATACCAGA	47.6	42.9	58.3	58.1	155 bp	1.0	60.8