Hotspot

rns-ompR

Genome · 28097-30127 · 2031 bp

Back to Chiharaea

Pi 0.0246

MarkerSeek score 60.80

Primer available yes

Label rns-ompR

Feature Metadata

label_name: rns-ompR
feature_type: igs
region: Genome
length_bp: 2031
variable_sites: 50
pi: 0.0246
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rns-ompR_p1	GAGCCAGGATCAAACTCTCC	CGAATACTTGTTTCGTCATCAACA	55.0	37.5	57.7	58.1	2106 bp	1.0	46.6
rns-ompR_p2	TGCATGTGTTAGGCATACCG	CGAATACTTGTTTCGTCATCAACA	50.0	37.5	58.3	58.1	2140 bp	1.0	46.5
rns-ompR_p3	AGCCAGGATCAAACTCTCCA	CGAATACTTGTTTCGTCATCAACA	50.0	37.5	58.3	58.1	2105 bp	1.0	46.5
rns-ompR_p4	TATTACTCACCCGTCCGCTA	CGAATACTTGTTTCGTCATCAACA	50.0	37.5	57.7	58.1	2185 bp	1.0	46.3
rns-ompR_p5	GAGCCAGGATCAAACTCTCC	ACTTGTTTCGTCATCAACAA	55.0	35.0	57.7	53.7	2101 bp	1.0	44.8