Hotspot

accD

Genome · 57578-59377 · 1800 bp

Back to Chesneya

Pi 0.261

MarkerSeek score 78.60

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: Genome
length_bp: 1800
variable_sites: 370
pi: 0.261
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	ATCGTCATTGATATGGGGCG	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.9	58.0	2894 bp	0.5	59.0
accD_p2	ATAGTTGTGTCCCTAGCGGT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.1	58.0	2418 bp	0.5	58.7
accD_p3	ATCGTCATTGATATGGGGCG	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.9	57.9	2900 bp	0.5	58.4
accD_p4	ATAGTTGTGTCCCTAGCGGT	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.1	57.9	2424 bp	0.5	58.1
accD_p5	CATAGTTGTGTCCCTAGCGG	CCATTGCAATTGCCGGAAAT	55.0	45.0	57.8	58.0	2419 bp	0.5	58.0