Hotspot

ndhB-ycf1

Genome · 108289-109036 · 748 bp

Back to Chesneya

Pi 0.129

MarkerSeek score 70.80

Primer available yes

Label ndhB-ycf1

Feature Metadata

label_name: ndhB-ycf1
feature_type: igs
region: Genome
length_bp: 748
variable_sites: 55
pi: 0.129
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.57
markerseek_score: 70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB-ycf1_p1	ATGCTTGCGTATTCGTCCAT	CCCTTCTTCCATAAACCGGG	45.0	55.0	58.0	57.9	1181-1483 bp	1.0	88.5
ndhB-ycf1_p2	GGACTGGCATCTTCTTCTGG	CCCTTCTTCCATAAACCGGG	55.0	55.0	58.0	57.9	1269-1571 bp	1.0	88.4
ndhB-ycf1_p3	GGACTGGCATCTTCTTCTGG	CCCCCTTCTTCCATAAACCG	55.0	55.0	58.0	57.9	1573 bp	0.5	59.3
ndhB-ycf1_p4	GGACTGGCATCTTCTTCTGG	CCCCTTCTTCCATAAACCGG	55.0	55.0	58.0	57.9	1572 bp	0.5	59.3
ndhB-ycf1_p5	ATGCTTGCGTATTCGTCCAT	CCCCTTCTTCCATAAACCGG	45.0	55.0	58.0	57.9	1484 bp	0.5	59.2