Hotspot

rpl23-ycf2

Genome · 86228-87306 · 1079 bp

Back to Chesneya

Pi 0.135

MarkerSeek score 76.20

Primer available yes

Label rpl23-ycf2

Feature Metadata

label_name: rpl23-ycf2
feature_type: igs
region: Genome
length_bp: 1079
variable_sites: 102
pi: 0.135
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.70
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl23-ycf2_p1	ATAGGTCGCCCTCTTCTACC	GCTAGGTCCAATGGAATGCA	55.0	50.0	58.0	57.9	1621 bp	0.5	59.5
rpl23-ycf2_p2	ATAGGTCGCCCTCTTCTACC	CTAGTGGACCCCTCAGTAGG	55.0	60.0	58.0	57.9	1851 bp	0.5	59.4
rpl23-ycf2_p3	ATAGGTCGCCCTCTTCTACC	CAAAATTCGGGGGTCCAAGA	55.0	50.0	58.0	58.1	1447 bp	0.5	59.3
rpl23-ycf2_p4	ATAGGTCGCCCTCTTCTACC	GAGTCCTCTGTGAATTGCGT	55.0	50.0	58.0	57.9	1481 bp	0.5	59.3
rpl23-ycf2_p5	ATAGGTCGCCCTCTTCTACC	GGGGGTCCAAGAGTTTGATC	55.0	55.0	58.0	57.9	1439 bp	0.5	58.9