Hotspot

rps12-psbB

Genome · 70387-71669 · 1283 bp

Back to Chesneya

Pi 0.211

MarkerSeek score 82.10

Primer available yes

Label rps12-psbB

Feature Metadata

label_name: rps12-psbB
feature_type: igs
region: Genome
length_bp: 1283
variable_sites: 265
pi: 0.211
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-psbB_p1	CCCTCTTAGTCCGTTCTTGC	ACCAAAACAAGCTACCCCTG	55.0	50.0	58.0	58.0	2107-2766 bp	1.0	88.9
rps12-psbB_p2	CCCTCTTAGTCCGTTCTTGC	ATCAAAACCTTCCACACCCC	55.0	50.0	58.0	58.0	2224-2883 bp	1.0	88.8
rps12-psbB_p3	AGACAAAGAAATGGCGGGTT	ACCAAAACAAGCTACCCCTG	45.0	50.0	58.0	58.0	2342 bp	0.5	59.9
rps12-psbB_p4	AGACAAAGAAATGGCGGGTT	ATCAAAACCTTCCACACCCC	45.0	50.0	58.0	58.0	2459 bp	0.5	59.9
rps12-psbB_p5	AGACAAAGAAATGGCGGGTT	ACACCCATATTCCAGGACCA	45.0	50.0	58.0	58.0	2391 bp	0.5	59.8