Hotspot

petA-psbJ

Genome · 62861-64021 · 1161 bp

Back to Chesneya

Pi 0.233

MarkerSeek score 88.60

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: Genome
length_bp: 1161
variable_sites: 223
pi: 0.233
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 88.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTCCAAGGTCTATTGT	AACTGGTATTCCCGTGATCG	45.0	50.0	56.5	57.4	1298-1349 bp	1.0	75.1
petA-psbJ_p2	TGAGAAGGTTCAATTGTCCGA	AACTGGTATTCCCGTGATCG	42.9	50.0	57.2	57.4	1220-1271 bp	1.0	73.1
petA-psbJ_p3	ACGTGTCCAAGGTCTATTGTT	AACTGGTATTCCCGTGATCG	42.9	50.0	57.2	57.4	1298-1349 bp	1.0	73.1
petA-psbJ_p4	ACGTGTCCAAGGTCTATTGT	ATTCCGGATTGGGATCATCC	45.0	50.0	56.5	57.1	1248-1299 bp	1.0	72.8
petA-psbJ_p5	TGAGAAGGTTCAATTGTCCGA	ATTCCGGATTGGGATCATCC	42.9	50.0	57.2	57.1	1170-1221 bp	1.0	70.8