Hotspot

psbK-trnQ-UUG

Genome · 56450-57063 · 614 bp

Back to Chesneya

Pi 0.11
MarkerSeek score 83.90
Primer available yes
Label psbK-trnQ-UUG

Feature Metadata

label_name
psbK-trnQ-UUG
feature_type
igs
region
Genome
length_bp
614
variable_sites
62
pi
0.11
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
83.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-trnQ-UUG_p1 AGCAGCTTGCCAAACAAAAG TGCTTTCTTCGCCAGTCTTT 45.0 45.0 58.1 58.0 1531 bp 0.5 59.3
psbK-trnQ-UUG_p2 AGCAGCTTGCCAAACAAAAG TTGCTTTCTTCGCCAGTCTT 45.0 45.0 58.1 58.0 1532 bp 0.5 59.3
psbK-trnQ-UUG_p3 CTTACAGCAGCTTGCCAAAC TGCTTTCTTCGCCAGTCTTT 50.0 45.0 57.9 58.0 1536 bp 0.5 59.3
psbK-trnQ-UUG_p4 CTTACAGCAGCTTGCCAAAC TTGCTTTCTTCGCCAGTCTT 50.0 45.0 57.9 58.0 1537 bp 0.5 59.3
psbK-trnQ-UUG_p5 AGCAGCTTGCCAAACAAAAG GAGTCTGAGTCTCAGTCGGA 45.0 55.0 58.1 57.9 1468 bp 0.5 58.7