Hotspot

atpH-atpF

Genome · 49705-50306 · 602 bp

Back to Chesneya

Pi 0.101

MarkerSeek score 85.10

Primer available yes

Label atpH-atpF

Feature Metadata

label_name: atpH-atpF
feature_type: igs
region: Genome
length_bp: 602
variable_sites: 58
pi: 0.101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 85.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpF_p1	ATTTGCGAATCCTTTTGTTT	GATGGCCAGTAACTCAAGCA	30.0	50.0	52.5	57.9	671-741 bp	1.0	50.0
atpH-atpF_p2	ATTTGCGAATCCTTTTGTTT	TGGCCAGTAACTCAAGCAAA	30.0	45.0	52.5	57.6	669-739 bp	1.0	50.0
atpH-atpF_p3	ATTTGCGAATCCTTTTGTTT	ATGGCCAGTAACTCAAGCAA	30.0	45.0	52.5	57.4	670-740 bp	1.0	50.0
atpH-atpF_p4	ATTTGCGAATCCTTTTGTTT	CGGATGGCCAGTAACTCAAG	30.0	55.0	52.5	58.3	673-743 bp	1.0	49.9
atpH-atpF_p5	ATTTGCGAATCCTTTTGTTT	ATCAAACCCGAAACTTCCGG	30.0	50.0	52.5	58.5	693-763 bp	1.0	49.7