Hotspot

trnG-GCC-psbZ

Genome · 25556-26238 · 683 bp

Back to Chesneya

Pi 0.0872
MarkerSeek score 81.50
Primer available yes
Label trnG-GCC-psbZ

Feature Metadata

label_name
trnG-GCC-psbZ
feature_type
igs
region
Genome
length_bp
683
variable_sites
53
pi
0.0872
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.89
markerseek_score
81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-psbZ_p1 CGTCTTCTCCTTGGCAAAGA AGTGGGTATTCTTAATTCTCTCA 50.0 34.8 57.8 54.5 761-896 bp 1.0 49.1
trnG-GCC-psbZ_p2 CGTCTTCTCCTTGGCAAAGA AGTGGGTATTCTTAATTCTCTCATCT 50.0 34.6 57.8 57.4 761-896 bp 1.0 49.1
trnG-GCC-psbZ_p3 CGTCTTCTCCTTGGCAAAGA TGGGTATTCTTAATTCTCTCATCTCT 50.0 34.6 57.8 57.2 759-894 bp 1.0 49.1
trnG-GCC-psbZ_p4 CGTCTTCTCCTTGGCAAAGA AGTGGGTATTCTTAATTCTCTCATC 50.0 36.0 57.8 56.2 761-896 bp 1.0 49.1
trnG-GCC-psbZ_p5 CGTCTTCTCCTTGGCAAAGA GTGGGTATTCTTAATTCTCTCATCT 50.0 36.0 57.8 56.2 760-895 bp 1.0 49.1