Hotspot

trnfM-CAU-trnG-GCC

Genome · 25333-25482 · 150 bp

Back to Chesneya

Pi 0.0902

MarkerSeek score 80.00

Primer available yes

Label trnfM-CAU-trnG-GCC

Feature Metadata

label_name: trnfM-CAU-trnG-GCC
feature_type: igs
region: Genome
length_bp: 150
variable_sites: 12
pi: 0.0902
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnfM-CAU-trnG-GCC_p1	GTAGCTCGCAAGGCTCATAA	TCTTTGCCAAGGAGAAGACG	50.0	50.0	57.8	57.8	237-257 bp	1.0	84.7
trnfM-CAU-trnG-GCC_p2	TAGCTCGCAAGGCTCATAAC	TCTTTGCCAAGGAGAAGACG	50.0	50.0	57.8	57.8	236-256 bp	1.0	84.7
trnfM-CAU-trnG-GCC_p3	TCTTCCGGAGTAGAGCAACT	TCTTTGCCAAGGAGAAGACG	50.0	50.0	57.8	57.8	279 bp	0.5	56.9
trnfM-CAU-trnG-GCC_p4	TCTTCCGGAGTAGAGCAACT	GCGGATGCGGATATAGTTGA	50.0	50.0	57.8	57.6	312 bp	0.5	55.3
trnfM-CAU-trnG-GCC_p5	TAGCTCGCAAGGCTCATAAC	GCGGATGCGGATATAGTTGA	50.0	50.0	57.8	57.6	289 bp	0.5	55.3