Hotspot

trnL-CAA-trnT-UGU

Genome · 14100-15740 · 1641 bp

Back to Chesneya

Pi 0.0851

MarkerSeek score 80.00

Primer available yes

Label trnL-CAA-trnT-UGU

Feature Metadata

label_name: trnL-CAA-trnT-UGU
feature_type: igs
region: Genome
length_bp: 1641
variable_sites: 105
pi: 0.0851
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.75
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-trnT-UGU_p1	TAGCGTCTACCAATTTCGCC	GCGATGCTCTAACCTCTGAG	50.0	55.0	58.1	57.9	1575-1700 bp	1.0	87.5
trnL-CAA-trnT-UGU_p2	TAGCGTCTACCAATTTCGCC	GGAATCGAACCGATGACCAT	50.0	50.0	58.1	57.8	1607-1732 bp	1.0	86.6
trnL-CAA-trnT-UGU_p3	AGTCCGTAGCGTCTACCAAT	GCGATGCTCTAACCTCTGAG	50.0	55.0	58.2	57.9	1581-1706 bp	1.0	86.1
trnL-CAA-trnT-UGU_p4	CCCATTGTGAATTCCAGGGT	GCGATGCTCTAACCTCTGAG	50.0	55.0	57.8	57.9	1660-1785 bp	1.0	85.9
trnL-CAA-trnT-UGU_p5	CCCATTGTGAATTCCAGGGT	GGAATCGAACCGATGACCAT	50.0	50.0	57.8	57.8	1692-1817 bp	1.0	85.0