Hotspot

trnV-UAC-ndhC

Genome · 10491-10925 · 435 bp

Back to Chesneya

Pi 0.0962

MarkerSeek score 81.10

Primer available yes

Label trnV-UAC-ndhC

Feature Metadata

label_name: trnV-UAC-ndhC
feature_type: igs
region: Genome
length_bp: 435
variable_sites: 40
pi: 0.0962
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-UAC-ndhC_p1	ATCAGAGCACAGGGAGTCAT	GCCTAGGACTAGATCGTGGT	50.0	55.0	58.1	58.0	1253-1255 bp	1.0	83.4
trnV-UAC-ndhC_p2	ATCAGAGCACAGGGAGTCAT	TGCCTAGGACTAGATCGTGG	50.0	55.0	58.1	58.0	1254-1256 bp	1.0	83.3
trnV-UAC-ndhC_p3	ATCAGAGCACAGGGAGTCAT	TTTCATAGTTACCGTGCCCG	50.0	50.0	58.1	58.0	1294-1296 bp	1.0	83.2
trnV-UAC-ndhC_p4	GCACAAGGGCTATAGCTCAG	TTTCATAGTTACCGTGCCCG	55.0	50.0	58.1	58.0	1745-1761 bp	1.0	83.1
trnV-UAC-ndhC_p5	GCACAAGGGCTATAGCTCAG	GCCTAGGACTAGATCGTGGT	55.0	55.0	58.1	58.0	1704-1720 bp	1.0	83.1