Hotspot

ycf1

SSC · 122843-128488 · 5646 bp

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Pi 0.0109

MarkerSeek score 50.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5646
variable_sites: 173
pi: 0.0109
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TATCCCAGGCTTCGGCTATT	TTTCGGCTACTTTTTCCGGA	50.0	45.0	58.3	57.5	186-192 bp	1.0	80.3
ycf1_p2	TATCCCAGGCTTCGGCTATT	TCGGCTACTTTTTCCGGATT	50.0	45.0	58.3	57.2	184-190 bp	1.0	78.6
ycf1_p3	TATCCCAGGCTTCGGCTATT	TTCGGCTACTTTTTCCGGAT	50.0	45.0	58.3	57.2	185-191 bp	1.0	78.5
ycf1_p4	TATCCCAGGCTTCGGCTATT	TTCGGCTACTTTTTCCGGATT	50.0	42.9	58.3	57.9	185-191 bp	1.0	76.2
ycf1_p5	TATCCCAGGCTTCGGCTATT	TTTCGGCTACTTTTTCCGGAT	50.0	42.9	58.3	57.9	186-192 bp	1.0	76.2