Hotspot

rrn4.5-rrn23

IRa · 130292-130383 · 92 bp

Back to Chenopodium

Pi 0.0401

MarkerSeek score 54.00

Primer available yes

Label rrn4.5-rrn23

Feature Metadata

label_name: rrn4.5-rrn23
feature_type: igs
region: IRa
length_bp: 92
variable_sites: 7
pi: 0.0401
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn4.5-rrn23_p1	TCACTGCACTTCCACTTGAC	AAGTAGTAAGCCCACCCCAA	50.0	50.0	58.0	58.2	186 bp	1.0	80.6
rrn4.5-rrn23_p2	CTGTGGGTCTGTTAGGATGC	AAGTAGTAAGCCCACCCCAA	55.0	50.0	58.0	58.2	220 bp	1.0	80.3
rrn4.5-rrn23_p3	CTGTTAGGATGCCTCAGCTG	AAGTAGTAAGCCCACCCCAA	55.0	50.0	58.0	58.2	212 bp	1.0	80.2
rrn4.5-rrn23_p4	GTCTGTTAGGATGCCTCAGC	AAGTAGTAAGCCCACCCCAA	55.0	50.0	58.1	58.2	214 bp	1.0	80.2
rrn4.5-rrn23_p5	CTGTGGGTCTGTTAGGATGC	TAGTAAGCCCACCCCAAGAT	55.0	50.0	58.0	57.7	217 bp	1.0	80.1