Hotspot

rpl32-trnL-UAG

SSC · 112227-113299 · 1073 bp

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Pi 0.0187

MarkerSeek score 65.40

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1073
variable_sites: 55
pi: 0.0187
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGTATTGGGCGGCATTAAGA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	1049-1206 bp	1.0	87.5
rpl32-trnL-UAG_p2	GGTATTGGGCGGCATTAAGA	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	1050-1207 bp	1.0	87.5
rpl32-trnL-UAG_p3	GGTATTGGGCGGCATTAAGA	CTCGCACTGCTTCCTAAGAG	50.0	55.0	58.0	58.1	1070-1227 bp	1.0	86.8
rpl32-trnL-UAG_p4	GGTATTGGGCGGCATTAAGA	CTCTCGCACTGCTTCCTAAG	50.0	55.0	58.0	58.1	1072-1229 bp	1.0	86.8
rpl32-trnL-UAG_p5	GGTATTGGGCGGCATTAAGA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	1059-1216 bp	1.0	86.5