Hotspot

ndhF-rpl32

SSC · 111093-112052 · 960 bp

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Pi 0.0168

MarkerSeek score 54.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 960
variable_sites: 48
pi: 0.0168
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TAGGGCGTGTGAACCAAAAA	TCTTAATGCCGCCCAATACC	45.0	50.0	57.9	58.0	1551-1561 bp	1.0	83.2
ndhF-rpl32_p2	ATTGGGCCGATTTAGCAACT	TCTTAATGCCGCCCAATACC	45.0	50.0	57.9	58.0	1773-1783 bp	1.0	82.4
ndhF-rpl32_p3	GGGGAAATTGGGCCGATTTA	TCTTAATGCCGCCCAATACC	50.0	50.0	58.2	58.0	1779-1789 bp	1.0	81.8
ndhF-rpl32_p4	AATAGGGCGTGTGAACCAAA	TCTTAATGCCGCCCAATACC	45.0	50.0	57.7	58.0	1553-1563 bp	1.0	81.4
ndhF-rpl32_p5	ATAGGGCGTGTGAACCAAAA	TCTTAATGCCGCCCAATACC	45.0	50.0	57.7	58.0	1552-1562 bp	1.0	81.4