Hotspot

psaJ-rpl33

LSC · 66432-66834 · 403 bp

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Pi 0.029

MarkerSeek score 43.80

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 403
variable_sites: 24
pi: 0.029
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ATGCGTTGACATTCCCCTTT	TCGCAATTCCAACCGACTAG	45.0	50.0	58.1	58.0	581-582 bp	1.0	83.3
psaJ-rpl33_p2	ACCCGTGACATTTTGTACCC	TCGCAATTCCAACCGACTAG	50.0	50.0	58.1	58.0	1150-1166 bp	1.0	82.3
psaJ-rpl33_p3	CCCGTGACATTTTGTACCCA	TCGCAATTCCAACCGACTAG	50.0	50.0	58.1	58.0	1149-1165 bp	1.0	82.3
psaJ-rpl33_p4	ACAGGATTTGAACCCGTGAC	TCGCAATTCCAACCGACTAG	50.0	50.0	58.1	58.0	1161-1177 bp	1.0	82.2
psaJ-rpl33_p5	CAGGATTTGAACCCGTGACA	TCGCAATTCCAACCGACTAG	50.0	50.0	58.1	58.0	1160-1176 bp	1.0	82.2