Hotspot

rps4-trnT-UGU

LSC · 45944-46335 · 392 bp

Back to Chenopodium

Pi 0.0302

MarkerSeek score 68.50

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 392
variable_sites: 30
pi: 0.0302
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	CCCCTAGACGGCGTATTTTT	GATGGTCATCGGTTCGAGTC	50.0	55.0	57.7	58.2	463-486 bp	1.0	83.6
rps4-trnT-UGU_p2	CCCCTAGACGGCGTATTTTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.7	57.9	494-517 bp	1.0	83.4
rps4-trnT-UGU_p3	AGATCACTTCCGGCTCTAGG	GATGGTCATCGGTTCGAGTC	55.0	55.0	58.3	58.2	509-532 bp	1.0	83.1
rps4-trnT-UGU_p4	AGATCACTTCCGGCTCTAGG	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.3	57.9	540-563 bp	1.0	82.9
rps4-trnT-UGU_p5	AGATCACTTCCGGCTCTAGG	CCGCTTAGCTCAGAGGTTAG	55.0	55.0	58.3	57.5	548-571 bp	1.0	79.9