Hotspot

ycf3-trnS-GGA

LSC · 43963-44896 · 934 bp

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Pi 0.0153

MarkerSeek score 50.60

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 934
variable_sites: 35
pi: 0.0153
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 50.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CATTTATCCGCGATCTAGGCA	CCCTCGGTAAACAAAAGCCT	47.6	50.0	58.0	58.1	1005-1023 bp	1.0	75.8
ycf3-trnS-GGA_p2	CATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	47.6	50.0	58.0	58.2	1026-1044 bp	1.0	75.3
ycf3-trnS-GGA_p3	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTGAA	47.6	45.0	58.0	57.8	976-994 bp	1.0	75.2
ycf3-trnS-GGA_p4	CATTTATCCGCGATCTAGGCA	GACGGAAAGAGAGGGATTCG	47.6	55.0	58.0	57.8	1027-1045 bp	1.0	74.8
ycf3-trnS-GGA_p5	CATTTATCCGCGATCTAGGCA	ACCCTCGGTAAACAAAAGCC	47.6	50.0	58.0	58.4	1006-1024 bp	1.0	73.5