Hotspot

psaA-ycf3

LSC · 41124-41933 · 810 bp

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Pi 0.0136

MarkerSeek score 51.20

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 810
variable_sites: 30
pi: 0.0136
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGAAAAATGACCGGGTCTGG	ATTGGTTGAAGATCACGGGG	50.0	50.0	58.1	57.9	939-952 bp	1.0	81.0
psaA-ycf3_p2	TTTTACTTCTGGTTCCGGCG	ATTGGTTGAAGATCACGGGG	50.0	50.0	58.5	57.9	873-886 bp	1.0	78.1
psaA-ycf3_p3	GTCTGGCCCATTCCTCAAAC	ATTGGTTGAAGATCACGGGG	55.0	50.0	58.8	57.9	925-938 bp	1.0	75.2
psaA-ycf3_p4	TGAAAAATGACCGGGTCTGG	GCTATAACGCTTACTCCTGGG	50.0	52.4	58.1	58.0	979-992 bp	1.0	73.7
psaA-ycf3_p5	TGAAAAATGACCGGGTCTGG	GGCTATAACGCTTACTCCTGG	50.0	52.4	58.1	58.0	980-993 bp	1.0	73.7