Hotspot

psbM-trnD-GUC

LSC · 28713-29498 · 786 bp

Back to Chenopodium

Pi 0.0117

MarkerSeek score 54.00

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 786
variable_sites: 24
pi: 0.0117
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	TCATTCTGGCTAACCGTTTT	TCAATTGGTTAGAGCACCGC	40.0	50.0	55.3	58.6	949-956 bp	1.0	55.8
psbM-trnD-GUC_p2	TCATTCTGGCTAACCGTTTTT	TCAATTGGTTAGAGCACCGC	38.1	50.0	56.1	58.6	949-956 bp	1.0	54.3
psbM-trnD-GUC_p3	AATCATTCTGGCTAACCGTT	TCAATTGGTTAGAGCACCGC	40.0	50.0	55.0	58.6	951-958 bp	1.0	53.9
psbM-trnD-GUC_p4	ATCATTCTGGCTAACCGTTT	TCAATTGGTTAGAGCACCGC	40.0	50.0	55.0	58.6	950-957 bp	1.0	53.9
psbM-trnD-GUC_p5	TCTGGCTAACCGTTTTTACA	TCAATTGGTTAGAGCACCGC	40.0	50.0	54.9	58.6	945-952 bp	1.0	53.0