Hotspot

trnH-GUG-psbA

LSC · 74-213 · 140 bp

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Pi 0.0276

MarkerSeek score 58.00

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 140
variable_sites: 11
pi: 0.0276
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CTAGACCTAGCCGCTATCGA	55.0	55.0	58.1	57.9	195-209 bp	0.857143	81.2
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	TCTAGACCTAGCCGCTATCG	55.0	55.0	58.1	57.9	196-210 bp	0.857143	81.2
trnH-GUG-psbA_p3	CAATCCACTGCCTTGATCCA	CTAGACCTAGCCGCTATCGA	50.0	55.0	57.9	57.9	209-223 bp	0.857143	80.9
trnH-GUG-psbA_p4	CAATCCACTGCCTTGATCCA	TCTAGACCTAGCCGCTATCG	50.0	55.0	57.9	57.9	210-224 bp	0.857143	80.9
trnH-GUG-psbA_p5	AATCCACTGCCTTGATCCAC	CTAGACCTAGCCGCTATCGA	50.0	55.0	57.9	57.9	208-222 bp	0.857143	80.9